Intracellular pathogens establish their infection by interacting with host cells and suppressing innate human defense systems. Our research focuses on the effector proteins (virulence factors) that Burkholderia spp., Francisella tularensis, and Coxiella burnetii shuttle into the host cytosol using different secretion systems and which host proteins they interact with. We infer these interactions using bioinformatics tools, verify them via in vitro yeast two-hybrid assays, and validate them using in vivo infectivity murine models.
Memisevic, V., K. Kumar, N. Zavaljevski, D. DeShazer, A. Wallqvist, and J. Reifman. DBSecSys 2.0: a database of Burkholderia mallei and Burkholderia pseudomallei secretion systems. BMC Bioinformatics. 2016 September 20; 17:387. [PDF 777 KB]
Wallqvist, A., V. Memisevic, N. Zavaljevski, R. Pieper, S. V. Rajagopala, K. Kwon, C. Yu, T. A. Hoover, and J. Reifman. Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors. BMC Genomics. 2015 December 29; 16:1106. [PDF 1492 KB]
Chiang, C. Y., I. Uzoma, D. J. Lane, V. Memisevic, F. Alem, K. Yao, S. Bavari, A. Wallqvist, R. M. Hakami, and R. G. Panchal. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Frontiers in Microbiology. 2015 July 27; 6:683. [PDF 5386 KB]
Memisevic, V., N. Zavaljevski, S. V. Rajagopala, K. Kwon, R. Pieper, D. DeShazer, J. Reifman, and A. Wallqvist. Mining host-pathogen protein interactions to characterize Burkholderia mallei infectivity mechanisms. PLOS Computational Biology. 2015 March 4; 11(3):e1004088. [PDF 1613 KB]
Memisevic, V., K. Kumar, L. Cheng, N. Zavaljevski, D. DeShazer, A. Wallqvist, and J. Reifman. DBSecSys: a database of Burkholderia mallei secretion systems. BMC Bioinformatics. 2014 July 16; 15:244. [PDF 1341 KB]
Memisevic, V., N. Zavaljevski, R. Pieper, S. V. Rajagopala, K. Kwon, K. Townsend, C. Yu, X. Yu, D. Deshazer, J. Reifman, and A. Wallqvist. Novel Burkholderia mallei virulence factors linked to specific host-pathogen protein interactions. Molecular & Cellular Proteomics. 2013 November 1; 12(11):3036-3051. [PDF 2010 KB]